Clone

Target

IDP00345  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 08, 2008  

Experiment End Date

Sep 09, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

678  

Reference Sequence Used

MASYGGKKRN NKKAIIIGSI VAIVVLAGIA IYFFIQNQHK DEKNALAAAE TFTSNIAKEK YDKLGGSVSS ESLKKVEFTK KEMEDKYQAV YDGIGAKNIK VKNLKSVYDD KENKFNLTYE LEMRTSLGKL ATQKYKTTIS KQDDDWKIDW KPALIFPGMV KTDKVRITED DAERGQIVDR NGNPLATTGQ FAEAGVVPSK LGEGDEKVKN IADISKKLEV STDYINKQLD QKWVQADSFV PLVTLNKDNL PEATGLTYAQ KEMRTYPLNE ATSHLIGYVG EVSAEDIEKN PKLSVGDVIG KSGLERYYDK QLRGKNGGAI KIINDQTKQE DTLQKIDKKD GEEIKLTIDA AVQKKAFDSL GSETGAVTMI NPTNGELLAL VSTPSYDANQ MVLGITSEDY AKYNDDKRLP FLARYANRYA PGSTFKTITA TIGLDTGITK PDKVREISGL QWQKDASWGK YFVTRVHDVP KVNMTDALVH SDNIYFAQEG LEIGKDKLTA GLKKFDFDKE YNLPFTMKPA QISNDGLNSE ILLADTSYGQ GELLMSPIQQ AIAYSAIAND GKMPYPKLDT KEKAGKETQA TEAKSANQVK AALVKTVSDP AGTAHALEIQ GHSIAAKTGT AELKEKQGED GLENGFVYAF DADNPNYLMV GMIENVKGRG GSGLVIDKLK PVIESMYK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCTAGTTATGGTGGGAAA  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTATACATACTTTCAATTACAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008