Clone

Target

IDP00908  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 08, 2008  

Experiment End Date

Sep 09, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

461  

Reference Sequence Used

MKDAQLNTLC QRHQAVLINS ASNSITVAVV DAPSHALLDA LHFATQKQID IVCWTRQQME NHRHKPDQAP SANAAKGGET AAQLLNQTLR SAMAKRASDI HLEPGASRYR IRLRIDGVLH ILQDIAKETG LALTARLKVL GNLDIAEHRL PQDGQFTVDL AGDSISFRIA TLPCKEGEKV VLRLLHQVEQ TLDLDTLGMY GAQLTAFRQA LQQPQGLVLV TGPTGSGKTV TLYSALQTRN TPGINLCSVE DPIEIPLDGI NQTQIHPRAG LTFQNVLRAL LRQDPDIIMV GEIRDGDTAE IAIKAAQTGH LVLSTLHTNS TSETLIRLQQ MGVARWMISS ALTLVVAQRL VRKLCPHCKQ RLSDPVVLSP NLWPSAIPRW QASGCQHCYH GFYGRTALFE VLTVTPALRQ LIASGASAQA LEAHLQQTGT GTLFENGCRA VEQGMTSFEE ILCVLGMPHE R  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAGACGCTCAGCTCAAC  

PCR Backward Primer

TTATCCACTTCCAATGTTAACGCTCATGGGGCATTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008