Clone

Target

IDP00220  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

464  

Reference Sequence Used

MSSSSFTTER PFTPELLSPA GTLKNMRYAF AYGADAVYAG QPRYSLRVRN NEFNHENLAL GIQEAHALGK RFYVVVNIAP HNSKLKTFLR DLKPVIEMGP DALIMSDPGL IMMVRDAFPQ MDIHLSVQAN AVNWATVKFW QQMGLTRVIL SRELSLEEIA EIRAQVPEME LEVFVHGALC MAYSGRCLLS GYINKRDPNQ GTCTNACRWE YNVQEGKEDD VGNIVHIHQP IAVKNVEPTL GIGAPTDKVF VIEEAQRPGE YMTASEDEHG TYIMNSKDLR AIQHVERLTQ LGVHSLKIEG RTKSFYYCAR TAQVYRRAID DAVAGKPFDP SLLTTLEGLA HRGYTEGFLR RHVHEEQQTY EYGYSVSERQ QFVGEFTGVR REGLAEVLVK NKFSRGDSVE LMTPKGNIQF TLESMQDKKG QPADIAPGNG HIMYLPIPEE IDVEYGLLIR NLNGTNSRNP HEKA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTCTTCATCATCATTTACAAC  

PCR Backward Primer

TTATCCACTTCCAATGTTACGCTTTTTCGTGGGGATT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008