Clone

Target
IDP00489  
Clone Suffix
2
Experiment Start Date
Jun 06, 2008  
Experiment End Date
Jun 07, 2008  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG19c  
Sequence Start
1  
Sequence End
282  
Reference Sequence Used
MRSQRELTSP MTPLENPGDI SDKPRLTTQK LSLGYGQQLI INKLSLSIKN GAFSVIIGPN GCGKSTLLRA LSRSLLPQNG SIRLDQQDIQ HYKAKVFARQ LSLLSQGASI SETLTVFDLV SRGRYAHQSF FHQWSTEDER IVKAAISAVN LESLVQQRVS ELSGGQRQRA WFAMTLAQHT PIVLLDEPTT YLDIAHQIEM LDLCQELQQQ GKTLVLVLHD INQALRYATH LIMLKEGQIY AEGLPESIVT EDSIAAVFGL RCRIITDPES GKPLVIPRRQ SR  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAGATCCCAAAGAGAGCTA  
PCR Backward Primer
TTATCCACTTCCAATGCTAACGCGACTGACGGCG  
Shipped To Lab
UT  
Shipped Date
Jul 16, 2008  
Inserted
Aug 08, 2008  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-09-15 Rosa Di University of Calgary Expression_protocol_Toronto success E. coli LB none production
2008-09-15 Rosa Di University of Calgary Expression_protocol_Toronto success E. coli LB none production
2008-06-16 2008-06-17 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli M9 minimal 1mM IPTG assay