Clone

Target

IDP00960  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Sep 08, 2008  

Experiment End Date

Sep 09, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

275  

Reference Sequence Used

MTLQQEIIQA LGAKPHINPE EEIRRSVDFL KAYLKTYPFL KSLVLGISGG QDSTLAGKLS QMAIAELREE TGDNALQFIA VRLPYGVQAD EQDCQDAIAF IQPDRVLTVN IKGAVLASEQ ALREAGIELS DFVRGNEKAR ERMKAQYSIA GMTHGVVVGT DHAAEAITGF FTKYGDGGTD INPLHRLNKR QGKQLLAALG CPEHLYKKVP TADLEDDRPS LPDEAALGVT YDNIDDYLEG KTLDPAIAKT IEGWYVKTEH KRRLPITVFD DFWKR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MTLQQEIIQA LGAKPHINPE EEIRRSVDFL KAYLKTYPFL KSLVLGISGG QDSTLAGKLS QMAIAELREE TGDNALQFIA VRLPYGVQAD EQDCQDAIAF IQPDRVLTVN IKGAVLASEQ ALREAGIELS DFVRGNEKAR ERMKAQYSIA GMTHGVVVGT DHAAEAITGF FTKYGDGGTD INPLHRLNKR QGKQLLAALG CPEHLYKKVP TADLEDDRPS LPDEAALGVT YDNIDDYLEG KTLDPAIAKT IEGWYVKTEH KRRLPITVFD DFWKR  

PCR Forward Primer

TACTTCCAATCCAATGCGATGACTCTGCAGCAAGAGATA  

PCR Backward Primer

TTATCCACTTCCAATGTTACCTTTTCCAAAAGTCGTCG  

Shipped To Lab

UT  

Shipped Date

Oct 09, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008