Clone

Target

IDP05409  

Clone Suffix

7

Sequence Comments

 

Experiment Start Date

May 02, 2013  

Experiment End Date

May 08, 2013  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG28  

Sequence Start

33  

Sequence End

852  

Reference Sequence Used

MVSNSKTTEV IIHYKEQSGN TKDWNLWIWG ENSSGKSYEF TGEDEFGKYA KINIDGDYNR LGFIIRTNEW EKDGGDRWIE NIKDGRAEVW ILSGDEKVYN SKPSSDLSIQ KATIDSFHEI TVTTNVPFNI KEKKIEMEGI KIKSISPYDI NSGDITNKVK IITEQKIDLK QTYKVKIENL ADTNTEIGKV IRSEEFDYLF YYGGNDLGNI YTPQHTKFRV WAPTASEAKL VTYKKWNDKI GTEINMQQGE KGTWKAELKG NQKGLYYTYK VKIGDKWTEA VDPYARAASV NGDKGAVVDL EETNPKKWNT NKKPKLKNPE DAIIYELHVR DLSIQPESGI KQKGKYLGVT EKGTKGPEGV KTGLDHMKDL GVTHVQLLPI FDYASVNEEK VNEPQYNWGY DPKNFNVPEG SYSTNPYEPT VRITELKQMI QTLHDNNLRV VMDVVYNHMY NAAESNFHKL VPGYYYRYNE DGTFANGTGV GNDTASERTM MRKFMIDSVT YWAKEYNLDG FRFDLMGIHD YETMNEIRKA VNQIDPSIIL HGEGWDLNTP LAAELKANQK NAEKMKGIAH FNDNIRDGLK GSVFEEKENG FVNGKENMED RIKKGITAGI DYDTNSSTYQ DPEQVLTYVE AHDNHTLWDK LELTNPGDSE EVRKQIHKLS SSILLTSQGI PFLHAGQEFM RTKYGDHNSY KSPDSINQMD WLRRATFNNE VDYMKGLIEL RKKYPAFRMT SAEQIKTHVS FIDAPKNTVT YTIEGNKHEY FTVAHNANRE AVEITLPSKG PWKVLVDGKQ AGSKPLYVVH DNKIKVPALS SIVLKSEKPI K  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGGTTTCAAATTCGAAAACAAC  

PCR Backward Primer

GGTTCTCCCCAGCTTTAATCGGTTTTTCTGATTTTAAA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jun 04, 2013