Clone

Target

IDP06241  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Aug 29, 2013  

Experiment End Date

Sep 04, 2013  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

262  

Reference Sequence Used

MAHLLEKTRK ITSILKRSEE QLQDELPYNA ITRQLADIIH CNACIINSKG RLLGYFMRYK TNTDRVEQFF QTKIFPDDYV QGANMIYETE ANLPVEHDMS IFPIESRDDF PDGLTTIAPI HVSGIRLGSL IIWRNDKKFE DEDLVLVEIA STVVGIQLLN FQREEDEKNI RRRTAVTMAV NTLSYSELRA VSAILGELNG NEGKLTASVI ADRIGITRSV IVNALRKLES AGIIESRSLG MKGTYLKVLI SDIFEEVKKR DY  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCACATTTATTAGAAAAAACT  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTAATCTCTTTTCTTCACTTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 18, 2013