Clone

Target
IDP06282  
Clone Suffix
1
Experiment Start Date
Aug 29, 2013  
Experiment End Date
Sep 04, 2013  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
failed  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
181  
Reference Sequence Used
MKKIAVFASG NGSNFQVIAE EFPVEFVFSD HRDAYVLERA KQLGVLSYAF ELKEFESKAD YEAALVELLE EHQIDLVCLA GYMKIVGPTL LSAYEGRIVN IHPAYLPEFP GAHGIEDAWN AGVGQSGVTI HWVDSGVDTG QVIKQVRVPR LADDTIDRFE ARIHEAEYRL YPEVVKALFT D  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAAAAAATAGCGGTTTTTGC  
PCR Backward Primer
TTATCCACTTCCAATGTCAATCTGTAAATAGAGCCTTC  

Related Expressions

 
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2013-09-09 2013-09-09 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay