Clone

Target

IDP92675  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Oct 08, 2013  

Experiment End Date

Oct 08, 2013  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG28  

Sequence Start

1  

Sequence End

688  

Reference Sequence Used

MLINATQKEE LRVALVDGQR LFDLDIESPG HESKKANIYK GRITRIEPSL EAAFVDYGAE RHGFLPLKEI AREYFPDGYS YQGRPNIKDV LNEGQEVIVQ IEKEERGSKG AALTAFISLA GSYLVLMPNN PRAGGISRRI EGDERTELKS ALSSLELPQG MGLIVRTAGV GKSAEELEWD LNVLLKHWSA IKQASDSNPA PFLIHQESNV IVRAIRDYLR RDIGEILIDS NSIYERALEH IRLVRPDFVN RVKKYEGEVP LFSHFQIESQ IESAFQREVR LPSGGSIVID PTEALTSIDI NSARATKGGD IEETALNTNL EAADEIARQL RLRDLGGLVV IDFIDMTPVR HQREVENRLR EAVRVDRARV QIGRISRFGL LEMSRQRLSP SLAEASHHIC PRCKGTGVIR DNESLALSVL RLIEEEALKD NTSQVLAVVP VSIASYLLNE KRRSINHIEK AQQVRITIVP NSDMETPHFE VIRVREGEEQ DVLSYLIPKK LEAMKEAEGK EVVDVELKPK RIEEPVLKGF AAPAEAVPAP TPKPKAEPQP VAEVQQPAQP GFFSRVFKAI ASLFSATPEA AKVEAPVTQN SEQDKPRRER NDRNNDRRRN PRDKNRRRGN EENNERNDKE TSNSNRKPQE RKPKQERGER NERNDRNERP ETERPERQIV VINEMKVKRL NCLSKVAN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGTTAATTAACGCAACTCAAAAA  

PCR Backward Primer

GGTTCTCCCCAGCATTGGCGACCTTGCTCAAGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 15, 2013