Clone

Target

IDP06077  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Dec 17, 2013  

Experiment End Date

Dec 20, 2013  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7-HaloV2  

Sequence Start

1  

Sequence End

159  

Reference Sequence Used

MQQAPVILST LDKLLNWGRS NSLWPLTYGL ACCAIEMMAT GGSRFDFDRF GTIFRASPRQ SDVMIIAGTL TKKHAEFMRR LYDQMPEPKW VISMGSCANT GGMFNTYATV QGADRVVPVD IYLPGCAPRP ETLQYALMVL QDKIRRSKAI KQDAPKRLV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCAACAAGCACCAGTTATTC  

PCR Backward Primer

TTATCCACTTCCAATGTCACACTAACCTTTTAGGAGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 14, 2014