Clone

Target

IDP00061  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 08, 2008  

Experiment End Date

Sep 09, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

279  

Reference Sequence Used

MSAKKERVDV LLVERGLIET REKAKRAIMA GLVYANEMRL DKPGEKIPQD TEITVKGQVM PYVSRGGYKL EKALETFHLD LQDKVMIDIG SSTGGFTDCA LQNGAKLSYA LDVGYNQLAW KLRQDERVVV MERTNFRYVT PADLERGLPQ FASIDVSFIS LKLILPVLKT MLMPNGDVAA LIKPQFEAGR EQVGKKGIVR DRKVHEAVVE MIVDFAIKEG YDVEGLTFSP ITGGDGNIEF LIHLKWHGER ENGENHSPVS VEQVVTEAHD VLKQKGKGE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGTGCAAAAAAAGAAAGAGT  

PCR Backward Primer

TTATCCACTTCCAATGTTATTCCCCTTTCCCTTTTTGT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008