Clone

Target

IDP00550  

Clone Suffix

2 - The clone is available in BEIR but it is out of stock.

Sequence Comments

142: K -> E,283: Q -> X  

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

664  

Reference Sequence Used

MTTELETSFA DLGLSAPILS ALTDLGYEKP SPIQLECIPH LLNGRDVLGM AQTGSGKTAA FGLPLLHNIK ADLKSPQVLV LAPTRELAVQ VAEALSSFSK HINGVNVVAL YGGQRYDVQL RALRQGPQVV VGTPGRLLDH LKRGTLNLSN LSGLVLDEAD EMLRMGFIED VETIMAQIPA EHQTALFSAT MPEAIRRITR RFMKEPQEVR IQSSVTTRPD ISQSYWRVGG GYRKNEALVR FLEAEDFDAA IIFVRTKNAT LEVAEALERS GYNSAALNGD MNQALREQTL ERLKDGRLDI LIATDVAARG LDVERISLVV NYDIPMDSES YVHRIGRTGR AGRAGRALLF VENRERRLLQ NIERTMKLTI PEVELPNADL LSERRLAKFA AKVSQQLESS DLDMYRALLA KLQPEEEFDI ETLAAALLKM AQGERPLILP PEAPRRPQRE FNSRDDRGND RGSRDRGDSR RDNRSDSRSA DSRDGGERPA RRERRDVGEM ELYRIEVGRD DGVEVRHIVG AIANEGDISS RYIGNIKLFA SHSTIELPKG MPGEMLSHFT RTRILNKPLN MQLLGDAQPH ERRERPAGGN GGERRGGGRP FSGERREGAG APPRRSFSGD RAPTGGGERR GNRDGQRSAA PRRDDAAAPA AAAAAPARRR FGDA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MTTELETSFA DLGLSAPILS ALTDLGYEKP SPIQLECIPH LLNGRDVLGM AQTGSGKTAA FGLPLLHNIK ADLKSPQVLV LAPTRELAVQ VAEALSSFSK HINGVNVVAL YGGQRYDVQL RALRQGPQVV VGTPGRLLDH LERGTLNLSN LSGLVLDEAD EMLRMGFIED VETIMAQIPA EHQTALFSAT MPEAIRRITR RFMKEPQEVR IQSSVTTRPD ISQSYWRVGG GYRKNEALVR FLEAEDFDAA IIFVRTKNAT LEVAEALERS GYNSAALNGD MNXALREQTL ERLKDGRLDI LIATDVAARG LDVERISLVV NYDIPMDSES YVHRIGRTGR AGRAGRALLF VENRERRLLQ NIERTMKLTI PEVELPNADL LSERRLAKFA AKVSQQLESS DLDMYRALLA KLQPEEEFDI ETLAAALLKM AQGERPLILP PEAPRRPQRE FNSRDDRGND RGSRDRGDSR RDNRSDSRSA DSRDGGERPA RRERRDVGEM ELYRIEVGRD DGVEVRHIVG AIANEGDISS RYIGNIKLFA SHSTIELPKG MPGEMLSHFT RTRILNKPLN MQLLGDAQPH ERRERPAGGN GGERRGGGRP FSGERREGAG APPRRSFSGD RAPTGGGERR GNRDGQRSAA PRRDDAAAPA AAAAAPARRR FGDA  

PCR Forward Primer

TACTTCCAATCCAATGCGATGACTACTGAGCTTGAAACC  

PCR Backward Primer

TTATCCACTTCCAATGTTATGCATCACCGAAGCGAC  

Shipped To Lab

UT  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008