Clone

Target
IDP06557  
Clone Suffix
143
Sequence Comments
IDP06557.21-254.P(261)..pMCSG73  
Experiment Start Date
May 15, 2015  
Experiment End Date
May 26, 2015  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG73  
Sequence Start
21  
Sequence End
254  
Reference Sequence Used
EIRSSVAEQE ADRDIIRFGE VSFSQLAEGV WMHTTYLDLM GFGPIPSNGL LVVNGDNTIL VDTAWTDEQT EQIVAWASMV LAKPVRAAVV THAHQDKMGG MAALHGANIA TWAHPLSNEL APEEGLVPAR NAITFDANGW ATGEAAQSLA PLRLYYPGGA HTRDNITVGL PELGIAFGGC MIKAGDASNL GNLADADTAA YAQSVRNFAA AFPDARTIAM SHSPPEGRKA IERT  
PCR Forward Primer
TACTTCCAATCCAATGCCGAAATTCGCTCCTCCGTGGCT  
PCR Backward Primer
TTATCCACTTCCAATGTTACGTGCGTTCGATTGCTTTACGG  
Shipped To Lab
ANL  
Shipped Date
May 26, 2015  
Inserted
Feb 19, 2015  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2015-02-19 2015-02-19 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2015-05-27 2015-05-27 Michael Endres Argonne National Laboratory standard expression_ANL failed E. coli LB + 40 mM K2HPO4 1 mM IPTG assay