Clone

Target

IDP95261  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jan 13, 2015  

Experiment End Date

Jan 13, 2015  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pCPD  

Sequence Start

1  

Sequence End

345  

Reference Sequence Used

MDQVNRPDLT PLKKASETVG QALKKGDIVV YESTVYPGAT EEVCIPILEK VSGLKFNQDF FAGYSPERIN PGDKVNTLTK IKKITSGSTP EVANTVDAVY ASIITAGTHK ASSIKVAEAA KVIENTQRDL NIALVNELSV IFDRIGIDTL DVLEAAGSKW NFLPFRPGLV GGHCIGVDPY YLTHKAEEVG YHPQVILAGR RINDNMARYV ARNTIKLMLQ NGIDVPRSKV GVLGVTFKEN CPDIRNSKVA DLIKELEFWG AQVVVADPWA DAEEVKHEYG VELGTVNAQN PVDSLIVAVG HSEFRSLSAS ELRSYVKAEK PVLADVKSLF DRTQMSDVGF TVFRL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGGACCAAGTTAACCGACC  

PCR Backward Primer

TGCTGCCCCGAGACGGAATACAGTAAAACC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 18, 2015