Clone

Target

IDP00583  

Clone Suffix

7

Sequence Comments

 

Experiment Start Date

May 14, 2015  

Experiment End Date

May 14, 2015  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

275  

Reference Sequence Used

MKNWIVGMVA LVTMVPVMAA TPWQKITHPV AGSPQSIGGF ANGCVIGAQP LPLESADYQV MRSDQRRYFG HPDLLNFIHR LSAQTHQQQL GTVLIGDMAM PAGGRFSSGH ASHQSGLDVD IWLQLPKQRW SQQQLLKPQP IDLVAVDGKR VIPALWQPQI ESLIKLAAKD NDVTRIFVNP AIKKQLCLDA GADRQWLHKV RPWFAHRAHM HVRLRCPANS LECVDQDTPP PGDGCGAELE SWFQPPPPSA KPGKTLPPPL PPSCQALLDN HFATE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAAATTGGATAGTGGGTAT  

PCR Backward Primer

TTATCCACTTCCAATGTTATTCCGTAGCGAAGTGGTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 16, 2015