Clone

Target

IDP01457  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Jul 27, 2015  

Experiment End Date

Jul 29, 2015  

Person

Olga Kiryukhina  

Lab

Northwestern University  

Protocol ID

NU_default_cloning  

Status

success  

Vector

pMCSG53  

Sequence Start

43  

Sequence End

237  

Reference Sequence Used

MKKKAPAKAS RVSPRVTTAE PAPIFIRWRF YLLLFFVLTA FCALVARVAY IQIIEPDNLI KEGDLRSIRA KTLQSARGII SDRNGEALAV SVPVEAVWAD PVAIYKEGGL VEKDRWYALA DVLGLDRQEM MKKIEDNRSR RFIYLQRQVS PAMAKYIREL KLAGIGLKEE SRRYYPAGEV SAHLVGVTGI DGHGLEGVER SFDSTLTGES GKSVTRKDKF GRVVENIALE EREEGKPIQL TIDQRLQAIA FRAVKQAMAD YRATSASAVM LDVKTGAVLA MVNAPSYNPN NRNDWQSFKM RNRVITDAFE PGSTVKPFVV LAALANGIAD KNTIIDTGDG TMQIGGSRVR DTSKVGKADL TLILKKSSNI GVAKLALEMP LEALLGMYSS VGLGEMSGLD LVGETIGIFP NRRRWSQFEI ATLSFGYGLA ITPIQLAHAY ATLGNHGKYQ PIHIIKGDQH GETRQVVDPK YVNQVLEMLE TVTQPGGTAT RAAVPGYRIA AKSGTSRKAI AGGYSDEYFA YTAGVAPVSD PRISLVVMVN EPQGDSYYGG AVAGPVFSEI MKGALQILNI APDENRFQNK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

ALVARVAYIQ IIEPDNLIKE GDLRSIRAKT LQSARGIISD RNGEALAVSV PVEAVWADPV AIYKEGGLVE KDRWYALADV LGLDRQEMMK KIEDNRSRRF IYLQRQVSPA MAKYIRELKL AGIGLKEESR RYYPAGEVSA HLVGVTGIDG HGLEGVERSF DSTLTGESGK SVTRKDKFGR VVENIALEER EEGKP  

PCR Forward Primer

TACTTCCAATCCAATGCGGCTTTGGTTGCACGAGTGG  

PCR Backward Primer

TTATCCACTTCCAATGTTAAGGCTTACCTTCTTCACG  

Shipped To Lab

NU  

Shipped Date

Jul 22, 2015  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 16, 2015