Clone

Target
IDP04682  
Clone Suffix
3
Experiment Start Date
Nov 05, 2015  
Experiment End Date
Nov 05, 2015  
Person
 
Lab
Northwestern University  
Protocol ID
NU_default_cloning  
Status
success  
Vector
pMCSG53  
Sequence Start
34  
Sequence End
186  
Reference Sequence Used
 
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
KLKVVTTYSI IYDMVKQIGG EKVEIHSLVP IGANPHEYDP LPKDVMKMTD ADMVLYNGLN LEEGGAWFKK LLKTANKSEK DAPVYKVSEG VEAIYLETKG LEKEPDPHAW MNIKNGILYA ENVKKALIKE DPKNKEFYTK NADNYVAELQ KLH  
PCR Forward Primer
TACTTCCAATCCAATGCGAAATTAAAAGTTGTAACTACATACT    
PCR Backward Primer
TTATCCACTTCCAATGTTAATGTAACTTTTGAAGTTCTGC    
Shipped To Lab
NU  
Shipped Date
Jul 22, 2015  
Inserted
Jun 19, 2015  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2015-06-16 2015-06-16 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2015-06-24 2015-06-24 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2015-11-05 2015-11-05 Albin Cardona-Correa Northwestern University NU_native_expression success E. coli Terrific broth/native media IPTG/Rhamnose production