Clone

Target

IDP00317  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Aug 05, 2015  

Experiment End Date

Aug 13, 2015  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

248  

Sequence End

759  

Reference Sequence Used

IKAPLTAKYH AATTQVKAPY MAEMVREAVV DQYGEEAYDR GLNVYTTISS KLQKEANRSL RDGLIAYDER HGYRKAQQNL GAFDRDIWLA ALKEKPIVDG ITPAAVTTIN SRSIVVLLPD GRAITVPWSG LSWARPALEN GYVGLAPRQA SDIVKVGDVV EVIQARNDQW WLTQIPEVEG GLVALNPQDG AVNALIGGFD YEHSNFNRIT QAERQPGSNF KPFLYSAALT KGYTLASMIN DAPVVMQDSG ENALWRPMND TRKFYGPTSI RMGLIKSRNL VSIRLLQKIG IPYAIEYLTR FGFDPNVLPH SLSLALGSAT LPPLQVASGY AIFANGGYRV TPYFISRIED QSHHVLFQAK PPRACSACIT NPNATAEQLP NPMAPQVITP QNAYLITQAM HDVIQYGTGR RAKVLNRPDL AGKTGTTNDQ VDAWFSGFNA NLETTVWVGY DNLRSLHEYG AQAALPIWIQ FMRAALQGQP EASLPQPPGM VMVRIDPHTG LLASPNQPDA RF  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATAAAAGCACCCCTCACCGC  

PCR Backward Primer

TTATCCACTTCCAATGTTAAAATCGCGCGTCTGGTTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 18, 2015