Clone

Target

IDP00256  

Clone Suffix

302

Sequence Comments

Added preM protein removed furin cleavage site and added TEV linker (AMQVSQNGDGWLLSPDQMDIGKTYKVGTGLCIFSSLDIGVPCNLTITYACVTLANTEEPVDLDCFCRDVQNVWVKYPLCKPGGSTLKSSLSITEHPSSVSLDKPTLWHHWNSVNDRIGKSEEWIVTNSSGENLYFQGLE).  

Experiment Start Date

Jan 21, 2009  

Experiment End Date

Jan 21, 2009  

Person

Chris Nelson  

Lab

Washington University  

Protocol ID

WU_basic_cloning_protocol_1  

Status

success  

Vector

pHLsec  

Sequence Start

1  

Sequence End

395  

Reference Sequence Used

TQCVNIQKRD IIRGASDVSW FDVLLEKGAC VTISAQDKPS VDLWLDDVIQ ESLIEGRHYC TKASITDLKA DARCPTQGEA TMREEHLDGY VCKRSFSDRG WGNGCGLFGK GSIVGCAKAT CGANDIIKSY SYDSPKVKYV IGAEVHQGKL LTNNSTDRIV KTTLTAESEK HTITIADYGS MDFTCRVVAS AELSNIRMIE LDGHMFNVHE DWLSDLPLPS KISGGSWHGM DRLVVFKEPH AVKMEIQECG DQRPAVFKSL VKAEEVTKTS NSYHLTGGHV DCRVSTLNLR MKGLTYQMCS SSFVWHKRPV ATQHGTVAME VKYKGSDAPC RIPVSVEKEG YNGKNFGNLI TANPFAANNE AVVFLELEAP LGVSTIKVGG AVFQWKQEGS SIGKA  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

GGC CTC GAG ACT CAG TGT GTC AAC ATC CAA  

PCR Backward Primer

ATGGGTACCAGCTTTTCCGATTGAACTTC  

Shipped To Lab

WU  

Shipped Date

Jan 21, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Feb 23, 2009