Clone

Target

IDP90139  

Clone Suffix

1

Sequence Comments

AgeI-KpnI clone. Remove signal peptide (MYKKLITFLF VIGAVA) added 6HIS tag at C-terminus.  

Experiment Start Date

Apr 20, 2009  

Experiment End Date

Apr 20, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

182  

Reference Sequence Used

MYKKLITFLF VIGAVASYSN NEYTPFNKLS VKLYIDGVDN IENSYTDDNN ELVLNFKEYT ISIITESCDV GFDSIDIDVI NDYKIIDMYT IDSSTIQRRE HTCRISTKLS CHYDKYPYIH KYEGDERQYS ITAEGKCYKG IKYEISMMND DTLLRKHTLK IGFTYIFDRH GHSNTYYSKY DF  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTATAAAAAACTAATAACGTTTTT  

PCR Backward Primer

TTATCCACTTCCAATGTTAAAAATCATATTTTGAATAATATGT  

Shipped To Lab

WU  

Shipped Date

Feb 07, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Feb 23, 2009