Clone

Target
IDP63265  
Clone Suffix
104
Sequence Comments
IDP63265.1-190.P(200)..pMCSG81  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG81  
Sequence Start
1  
Sequence End
190  
Reference Sequence Used
MRITLLDNFD SFTYNLVEQF CLLGAEVRVM RNDTPLPTIQ AALLADGCEL LVLSPGPGRP EDAGCMLELL AWARGRLPVL GVCLGHQALA LAAGGAVGEA RKPLHGKSTS LRFDQRHPLF DGIADLRVAR YHSLVVSRLP EGFDCLADAD GEIMAMADPR NRQLGLQFHP ESILTTHGQR LLENALLWCG  
PCR Forward Primer
GGAGTAAAGATAATGGGGGTTGGCTATCGACTGGAA  
PCR Backward Primer
GTGATGGTGATGATGGCCGCACCAGAGTAGAGCGTT  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-27 2016-05-27 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2016-05-21 2016-05-23 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production