Clone

Target
IDP56840  
Clone Suffix
103
Sequence Comments
IDP56840.1-180.F..pMCSG87  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG87  
Sequence Start
1  
Sequence End
180  
Reference Sequence Used
MEIGKRIKNL RLSKNLTQEE LGERTDLTKG YISQLERDLS SPSIETLFAI LEVLGSTPKD FFDEEEHNQK VIYGELEHTF FEDEEKGYRI KWLVPESNEK EMEPVLLEIE ANSCFKSFEP SLSETFAYVL KGEVTVLLGR NEYVAKKGEA IYFHAADEHQ IINRSSEHAT LILVATESYL  
PCR Forward Primer
CATCATCACCATCACCATATGGAAATTGGCAAACGCATTAAAAATCTTAGG  
PCR Backward Primer
ATGGTGATGGTGATGATGCATATGTATATCTCCTTCTTAAAGTTAAAC  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-12 2016-05-12 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2016-05-22 2016-05-24 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production