Clone

Target
IDP63170  
Clone Suffix
103
Sequence Comments
IDP63170.16-288.P(585)..pMCSG73  
Experiment Start Date
May 12, 2016  
Experiment End Date
May 12, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG73  
Sequence Start
16  
Sequence End
288  
Reference Sequence Used
DNTALFYRHW PTLLPGAKKV IVLFHRGHEH SGRLQHIVDE LAMPDTAFYA WDARGHGQTS GPRGYSPSLA RSVQDVDEFV RFAASDSQVG LEEVVVIAQS VGAVMVATWV HDYAPAIRGL VLASPAFKVK LYVPLARPGL ALWHRLRGLF FINSYVKGRY LTHDRQRVAS FNNDPLITRA IAVNILLDLY KTSERIVSDA AAITLPTQLL ISGDDYVVHR QPQIDFYQRL RSPLKELHLL PGFYHDTLGE ENRAQAFEKM QSFISRLYAN KSQ  
PCR Forward Primer
TACTTCCAATCCAATGCCGATAATACAGCGTTGTTTTATCGGCACT  
PCR Backward Primer
TTATCCACTTCCAATGTTATTGCGACTTGTTAGCATATAAACGGCTG  
Shipped To Lab
ANL  
Shipped Date
May 27, 2016  
Inserted
May 31, 2016  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2016-05-12 2016-05-12 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay
2016-05-23 2016-05-25 Gyorgy Babnigg Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production