Clone

Target

IDP07615  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 15, 2016  

Experiment End Date

Jun 15, 2016  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

394  

Reference Sequence Used

MLSNQTIEIV KATAPLIAET GPKLTAHFYD RMFTHNPELK DIFNMSNQRN GDQREALFNA ICAYAANIEN LPALLGAVEK IAHKHTSFLI TKDQYQIVGK HLIATIDELF NPGQEVLDAW AEAYGVLANV FIQREEQIYQ ANASQEGGWR GLREFELVGK QLESEHICSF VFKPTDGSKV TKYKPGQYLG IYINSDKFEN QEIRQYSLSS SVQENTYRIS VKREQGGKVS NYLHDELNIG DKVKLAAPAG DFFMDVDTNT PVVLISAGVG LTPTLSMLES LTEHHAPVTW VHATENSKHH AFKEHVNQLV TAKENMNALI WYNQPTAEDK IGEDFHFTGF VNLHEIEAAL KQDNVQVYFC GPVGFMQHVA KQLQELGVPQ EQFHYECFGP HKVV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MLSNQTIEIV KATAPLIAET GPKLTAHFYD RMFTHNPELK DIFNMSNQRN GDQREALFNA ICAYAANIEN LPALLGAVEK IAHKHTSFLI TKDQYQIVGK HLIATIDELF NPGQEVLDAW AEAYGVLANV FIQREEQIYQ ANASQEGGWR GLREFELVGK QLESEHICSF VFKPTDGSKV TKYKPGQYLG IYINSDKFEN QEIRQYSLSS SVQENTYRIS VKREQGGKVS NYLHDELNIG DKVKLAAPAG DFFMDVDTNT PVVLISAGVG LTPTLSMLES LTEHHAPVTW VHATENSKHH AFKEHVNQLV TAKENMNALI WYNQPTAEDK IGEDFHFTGF VNLHEIEAAL KQDNVQVYFC GPVGFMQHVA KQLQELGVPQ EQFHYECFGP HKVV  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTCAGCAATCAAACCATTG  

PCR Backward Primer

TTATCCACTTCCAATGTTAAACCACCTTATGTGGGCC  

Shipped To Lab

UT  

Shipped Date

Jul 12, 2016  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jul 15, 2016