Clone

Target
IDP95506  
Clone Suffix
3
Experiment Start Date
Apr 26, 2017  
Experiment End Date
Apr 28, 2017  
Person
Olga Kiryukhina  
Lab
Northwestern University  
Protocol ID
NU_default_cloning  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
115  
Reference Sequence Used
MADVAESQEN APAERAELTV PEMRQWLRNW VGKAVGKAPD SIDESVPMVE LGLSSRDAVA MAADIEDLTG VTLSVAVAFA HPTIESLATR IIEGEPETDL AGDDAEDWSR TGPAE  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MADVAESQEN APAERAELTV PEMRQWLRNW VGKAVGKAPD SIDESVPMVE LGLSSRDAVA MAADIEDLTG VTLSVAVAFA HPTIESLATR IIEGEPETDL AGDDAEDWSR TGPAE  
PCR Forward Primer
TACTTCCAATCCAATGCGATGGCTGACGTAGCGGAATC  
PCR Backward Primer
TTATCCACTTCCAATGTTACTCGGCCGGGCCGGT  
Shipped To Lab
NU  
Shipped Date
Apr 25, 2017  
Inserted
Mar 27, 2017  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2017-02-21 2017-02-21 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2017-07-10 2017-07-12 Olga Kiryukhina Northwestern University NU_SelMet_expression success E. coli SeMet IPTG production