Clone

Target
IDP96151  
Clone Suffix
1
Experiment Start Date
May 05, 2017  
Experiment End Date
May 05, 2017  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
307  
Reference Sequence Used
MNNLPLLNDL RVFMLVARRA GFAAVAEELG VSPAFVSKRI ALLEQTLNVV LLHRTTRRVT ITEEGERIYE WAQRILQDVG QMMDELSDVR QVPQGMLRII SSFGFGRQVV APALLALAKA YPQLELRFDV EDRLVDLVNE GVDLDIRIGD DIAPNLIARK LATNYRILCA SPEFIAQHGA PKHLTDLSAL PCLVIKERDH PFGVWQLRNK EGPHAIKVTG PLSSNHGEIV HQWCLDGQGI ALRSWWDVSE NIASGHLVQV LPEYYQPANV WAVYVSRLAT SAKVRITVEF LRQYFAEHYP NFSLEHA  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAATAATTTACCGCTGCTGA  
PCR Backward Primer
TTATCCACTTCCAATGTCAGGCATGCTCCAGTGAAA  

Related Expressions

 
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2017-05-05 2017-05-05 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay