Clone

Target

IDP96160  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

May 05, 2017  

Experiment End Date

May 05, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

472  

Reference Sequence Used

MKQEVILVLD CGATNVRAIA VNRQGKIVAR ASTPNASDIA MENNTWHQWS LDAILQRFAD CCRQINSELT ECHIRGIAVT TFGVDGALVD KQGNLLYPII SWKCPRTAAV MDNIERLISA QRLQAISGVG AFSFNTLYKL VWLKENHPQL LERAHAWLFI SSLINHRLTG EFTTDITMAG TSQMLDIQQR DFSPQILQAT GIPRRLFPRL VEAGEQIGTL QNSAAAMLGL PVGIPVISAG HDTQFALFGA GAEQNEPVLS SGTWEILMVR SAQVDTSLLS QYAGSTCELD SQAGLYNPGM QWLASGVLEW VRKLFWTAET PWQMLIEEAR LIAPGADGVK MQCDLLSCQN AGWQGVTLNT TRGHFYRAAL EGLTAQLQRN LQMLEKIGHF KASELLLVGG GSRNTLWNQI KANMLDIPVK VLDDAETTVA GAALFGWYGV GEFNSPEEAR AQIHYQYRYF YPQTEPEFIE EV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAACAAGAAGTTATCCTGG  

PCR Backward Primer

TTATCCACTTCCAATGTCACACTTCCTCTATAAATTCAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jun 17, 2017