Clone

Target

IDP00596  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

356  

Reference Sequence Used

MSGKTKRLMV MAGGTGGHVF PGLAVAHHLM AQGWQVRWLG TADRMEASLV PQHGIEIDFI KISGLRGKGL MAQLTAPIRI YRAVRQAQKI MRDYQPNVVL GMGGYVSGPG GLAAWLCGVP VVLHEQNGIA GLTNRWLARI AKKVLQAFPG AFPNADVVGN PVRTDVLALP LPAVRLSGRE GPIRVLVIGG SQGARILNQT LPLVAASLGE QITLWHQVGK GALPEVSQAY QQAGQAGHLV VEFIDDMAAA YAWADVVVCR SGALTVSEVA AAGLPAIFVP FQHKDRQQYW NALPLEKAGA AKIIEQPQFT ATSVSSLLAS WDRATLLSMA ERARSVAIPD ATERVAAEVV AASKSA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGTGGGAAGACCAAGCG  

PCR Backward Primer

TTATCCACTTCCAATGTTACGCTGACTTACTGGCCG  

Shipped To Lab

ANL  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008