Clone

Target

IDP96261  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 28, 2017  

Experiment End Date

Jul 28, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

106  

Sequence End

464  

Reference Sequence Used

LSPWGNASVD VKVDNEGHFT GSRGSWFVPL QDNDRYLTWS QLGLTQQDNG LVSNVGVGQR WARGNWLVGY NTFYDNLLDE NLQRAGFGAE AWGEYLRLSA NFYQPFAAWH EQTATQEQRM ARGYDLTARM RMPFYQHLNT SVSLEQYFGD RVDLFNSGTG YHNPVALSLG LNYTPVPLVT VTAQHKQGES GENQNNLGLN LNYRFGVPLK KQLSAGEVAE SQSLRGSRYD NPQRNNLPTL EYRQRKTLTV FLATPPWDLK PGETVPLKLQ IRSRYGIRQL IWQGDTQILS LTPGAQANSA EGWTLIMPDW QNGEGASNHW RLSVVVEDNQ GQRVSSNEIT LTLVEPFDAL SNDELRWEP  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGCTATCACCGTGGGGAAATGC  

PCR Backward Primer

TTATCCACTTCCAATGTTACGGTTCCCAGCGCAGT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 31, 2017