Clone

Target

IDP96281  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 28, 2017  

Experiment End Date

Jul 28, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

120  

Sequence End

441  

Reference Sequence Used

DLMPKRLAMI APEKIAISVI NPIQIFIKIC KPLAWGINAI ANLLFRLFKV NTTREDNITF DDISAVMDAG AQAGVLQKQE HHFIENVFEL EERTVPSSMT TRENVVYFTL NEHEDSIRQK LAEYPYSKFL VCNENIDQVI GYVDAKDILV RILNNQSLTQ LNESTIRTVL MIPDTLTLSE LLDRFRSTKE KFAVVINEYA LVVGVITLSD IMITVMGDWV TPMEEEQQII KRDNNSWLID GSTPIEDLKH ALEMDELPDE ENYETLAGFM MYRLRKIPRP ADFVEFGGYK FEVVDVDHFK IDQLLVTRVL EKNDLPSPPD EN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGGATTTAATGCCAAAACGCTTGG  

PCR Backward Primer

TTATCCACTTCCAATGTCAATTTTCATCAGGTGGAGAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 31, 2017