Clone

Target

IDP96401  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 28, 2017  

Experiment End Date

Jul 28, 2017  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

816  

Reference Sequence Used

MKKIKLVMVG NGMAGVRTLE ELLKLNSDFY DITVFGAEPH PNYNRILLSP VLAGEQTFEE IVLNDLNWYA ENGIKLLLDR KVVQIDRLRR RVVAADGSEA EYDRLLLATG SLPFILPIPG NRLQGVIGYR DIADTQAMID CARTHSHAVV IGGGLLGLEA ANGLKQRGMD VTVVHLSDWL LERQLDRTAG KLLQGALEAR GIRFRLNTQT QELMDNGSGR VCAVQFNDGD VIPADLVVMA AGIRPNTELA ESAGIPCNRG ILVNDTLQTY DPRIYAVGEC ANHRGIAYGL VAPLFEQAKV CANHLAHLGY ARYQGSVTST KLKVTGIDLF SAGDFIGGEG SETITLSDPI GGVYKKLVIK DDVLVGACLY GDTADGGWYF RQIRENHNVA QIRDHLMFGE NALGDVGHQG QNSAASMPDT AEVCGCNGVC KGTIVKAIQE HGLFSVDEVK KHTKAASSCG SCAGLVEQIL ISTVGGAADV KPKSEKAICG CSDLNHGQIR KAIREHRLTS LDAAMRFMDW KTPNGCATCR PALNYYLIST WPGEAKDDPQ SRLINERAHA NIQKDGTYSV VPRMWGGVTN AAELRRIADV ADKYQVPMVK VTGGQRIDLL GVKKDDLPAI WKDLDMPSGH AYGKSIRTVK TCVGSEFCRF GTQNSTQLGI DLEHDLFNMW SPHKVKLAVS GCPRNCAEAG IKDVGIIGVD SGWEMYIGGN GGIKTEVAEF FVKLKTAEEV REYNGAFLQL YREEAFYLER TVHYLQRVGM EHIRKAVVED AENRKALNDR LQFALSFEQD PWKQRIEQPQ LKKEFERISL KQLENV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAGAAGATCAAGCTGGTG  

PCR Backward Primer

TTATCCACTTCCAATGTCAGACGTTTTCCAGTTGCTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 31, 2017