Clone

Target
IDP96139  
Clone Suffix
103
Sequence Comments
IDP96139.31-205.P(205)..pMCSG53  
Experiment Start Date
Jun 04, 2018  
Experiment End Date
Jun 04, 2018  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG53  
Sequence Start
31  
Sequence End
205  
Reference Sequence Used
IQANTNLAIA DGARQQIGST LFYDPAYVQL TYPGGDVPQE RGVCSDVVIR ALRSQKVDLQ KLVHEDMAKN FAEYPQKWKL KRPDSNIDHR RVPNLETWFS RHDKTRPTSK NPSDYQAGDI VSWRLDNGLA HIGVVSDGFA RDGTPLVIHN IGAGAQEEDV LFNWRMVGHY RYFVK  
PCR Forward Primer
TACTTCCAATCCAATGCCCAAGGTAAGATTAAAGTCAGTCTCCCTAA  
PCR Backward Primer
TTATCCACTTCCAATGTTACTGAGCTTGCGTGGAGATGGA  
Shipped To Lab
ANL  
Shipped Date
Jan 15, 2019  
Inserted
Jun 09, 2018  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2018-06-04 2018-06-04 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay
2018-06-19 2018-06-19 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay
2019-01-16 2019-01-18 Christine Tesar Argonne National Laboratory standard expression_ANL success E. coli M9 minimal + semet 0.5 mM IPTG production