Clone

Target

IDP02549  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jan 28, 2009  

Experiment End Date

Jan 29, 2009  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

162  

Reference Sequence Used

MGVHVVLYQP EIPANTGNIA RTCAATGTEL HLIRPLGFST DDKMLKRAGL DYWQHVKITY YDSIEEFYEK NKDGEFFYLT KYGEKAHTAF DYSKREKDYY FVFGRETNGL PANVIEENFD HCLRIPMTDK VRSLNLSNTA AILIYEAFRQ QNYPGLDLEI VY  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGGAGTACATGTTGTTTTATA  

PCR Backward Primer

TTATCCACTTCCAATGTTAATAAACAATTTCTAAATCTAATC  

Shipped To Lab

ANL  

Shipped Date

Feb 19, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Feb 24, 2009