Clone

Target
IDP97897  
Clone Suffix
103
Sequence Comments
IDP97897.1-179.F..pMCSG53  
Experiment Start Date
Sep 27, 2019  
Experiment End Date
Oct 18, 2019  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
179  
Reference Sequence Used
MEDFLKLVAS RQSDRAYDMS RPVEPEKLER ILEAARLAPS ACNAQPWKFV VINDPELSVK VGKATAGLGM NKFAKDAPVH ILVVEESMNV TSFLGAKIKD KYFPLIDIGI ATAHITLAAE SEGLGSCILG WFDEKEIKKL TGIPSSKRLL LVITIGYSVK EKRKKARKPK EKVVSYNKY  
PCR Forward Primer
TACTTCCAATCCAATGCCATGGAAGATTTTCTGAAGTTAGTTGCTTCTC  
PCR Backward Primer
TTATCCACTTCCAATGTTAGTATTTATTGTAAGAAACCACCTTTTCTTTCGG  
Shipped To Lab
ANL  
Shipped Date
Oct 18, 2019  
Inserted
Oct 21, 2019  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2019-10-18 2019-10-18 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay
2019-12-21 2019-12-23 Natalia Maltseva Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 0.5 mM IPTG production