Clone

Target
IDP97898  
Clone Suffix
103
Sequence Comments
IDP97898.1-217.F..pMCSG53  
Experiment Start Date
Sep 27, 2019  
Experiment End Date
Oct 18, 2019  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
217  
Reference Sequence Used
MDIISVALKR HSTKAFDPAK KLTADEAEKI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGTYVF NERKMLDASH VVVFCAKTAM DDAWLERVVD QEEADGRFNT PEAKAANHKG RTYFADMHRV DLKDDDQWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDEEFGLKE KGFTSLVVVP VGHHSVEDFN ATLPKSRLPL STIVTEC  
PCR Forward Primer
TACTTCCAATCCAATGCCATGGATATTATTTCTGTCGCCCTGAAAC  
PCR Backward Primer
TTATCCACTTCCAATGTTAGCACTCAGTCACAATCGTGCTCA  
Shipped To Lab
ANL  
Shipped Date
Oct 18, 2019  
Inserted
Oct 21, 2019  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2022-02-25 2022-02-27 Natalia Maltseva Argonne National Laboratory standard expression_ANL success E. coli LB 0.5 mM IPTG production
2019-10-18 2019-10-18 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay
2019-10-15 2019-10-17 Natalia Maltseva Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 0.5 mM IPTG production