Clone

Target
IDP98044  
Clone Suffix
102
Sequence Comments
IDP98044.24-489.P(489)..pMCSG53  
Experiment Start Date
Aug 21, 2020  
Experiment End Date
Aug 21, 2020  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG53  
Sequence Start
24  
Sequence End
489  
Reference Sequence Used
QQQAGTTLNQ VVEQTLLSNP EIQAKYHDFR ASMEGQNVAR GGLRPQVNAQ GFVGKEWRSN VPDRGSYNWS RPGYSVELRQ LIFDGFRTSN DVKQAGYDKL GRFYELLATS DSTAYSAVQA YVDLQRYREM ELLARQNYSL HEDTLKQIRE RAESGVGRRV DLEQAGGRLA LAQTNLMTES ANLIDVQQRF QRITGMTPAV DLQPVPDVAG KLPQQPANFD DSLRMNPSFL AKQAYLQAAN AGVASAKGAF SPKFEFVAAT GRDREQPEPQ YRDVQSSSVQ LVMSYNLYRG GADSARVRQT AEQSYAARDV RDYTCRNVQQ DLAVAWNNIV RLREQIPFLR DHEIATAKVR NAYREQFQIG QRSLLDLLDT ENELFESRRA LANGMADLQL AQYRWLALSH KILPALSVQP ARDEMPEENA VLETTDEVLK LCNSPVPDVS RLAPVQVIYN SGAKPPTFVP ADKARP  
PCR Forward Primer
TACTTCCAATCCAATGCCCAGCAACAGGCCGGTACCAC  
PCR Backward Primer
TTATCCACTTCCAATGTTAGGGCCGGGCCTTGTCG  
Shipped To Lab
ANL  
Shipped Date
Sep 03, 2020  
Inserted
Aug 24, 2020  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2020-09-21 2020-09-23 Christine Tesar Argonne National Laboratory standard expression_ANL success E. coli M9 minimal + semet 0.5 mM IPTG production
2020-09-03 2020-09-03 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay