Clone

Target
IDP52003  
Clone Suffix
671
Sequence Comments
IDP52003.816-1060.P(1945)..pMCSG53  
Experiment Start Date
Nov 22, 2021  
Experiment End Date
Nov 22, 2021  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
failed  
Vector
pMCSG53  
Sequence Start
816  
Sequence End
1060  
Reference Sequence Used
YYHTTDPSFL GRYMSALNHT KKWKYPQVNG LTSIKWADNN CYLATALLTL QQIELKFNPP ALQDAYYRAR AGEAANFCAL ILAYCNKTVG ELGDVRETMS YLFQHANLDS CKRVLNVVCK TCGQQQTTLK GVEAVMYMGT LSYEQFKKGV QIPCTCGKQA TKYLVQQESP FVMMSAPPAQ YELKHGTFTC ASEYTGNYQC GHYKHITSKE TLYCIDGALL TKSSEYKGPI TDVFYKENSY TTTIK  
PCR Forward Primer
TACTTCCAATCCAATGCCTACTACCACACAACTGATCCTAGTTTTCT  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTTATGGTTGTTGTGTAACTGTTTTCTTTGTAGAAAA  
Shipped To Lab
ANL  
Shipped Date
Dec 09, 2021  
Inserted
Nov 29, 2021  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2021-12-10 2021-12-12 Christine Tesar Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 0.5 mM IPTG production
2021-12-09 2021-12-09 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB 1 mM IPTG assay