Clone

Target
IDP98885  
Clone Suffix
117
Sequence Comments
IDP98885.2-165.P(165)..pMCSG53  
Experiment Start Date
Apr 01, 2021  
Experiment End Date
Apr 02, 2021  
Person
Michael Endres  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG53  
Sequence Start
2  
Sequence End
165  
Reference Sequence Used
GWDLTVKMLA GNEFQVSLSS SMSVSELKAQ ITQKIGVHAF QQRLAVHPSG VALQDRVPLA SQGLGPGSTV LLVVDKCDEP LSILVRNNKG RSSTYEVRLT QTVAHLKQQV SGLEGVQDDL FWLTFEGKPL EDQLPLGEYG LKPLSTVFMN LRLRGGGTEP GGRS  
PCR Forward Primer
TACTTCCAATCCAATGCCGGCTGGGACCTGACGGTGAA  
PCR Backward Primer
TTATCCACTTCCAATGTTAGCTCCGCCCGCCAGG  
Shipped To Lab
ANL  
Shipped Date
Dec 07, 2021  
Inserted
Dec 13, 2021  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2020-11-10 2020-11-12 Christine Tesar Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 0.5 mM IPTG production
2021-04-05 2021-04-05 Michael Endres Argonne National Laboratory standard expression_ANL success E. coli LB + 40 mM K2HPO4 1 mM IPTG assay