Clone

Target

IDP00794  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

744  

Reference Sequence Used

MAKQKIKIKK NKIGAVLLVG LFGLLFFILV LRISYIMITG HSNGQDLVMK ANEKYLVKNA QQPERGKIYD RNGKVLAEDV ERYKLVAVID KKASANSKKP RHVVDKKETA KKLSTVINMK PEEIEKRLSQ KKAFQIEFGR KGTNLTYQDK LKIEKMNLPG ISLLPETERF YPNGNFASHL IGRAQKNPDT GELKGALGVE KIFDSYLSGS KGSLRYIHDI WGYIAPNTKK EKQPKRGDDV HLTIDSNIQV FVEEALDGMV ERYQPKDLFA VVMDAKTGEI LAYSQRPTFN PETGKDFGKK WANDLYQNTY EPGSTFKSYG LAAAIQEGAF DPDKKYKSGH RDIMGSRISD WNRVGWGEIP MSLGFTYSSN TLMMHLQDLV GADKMKSWYE RFGFGKSTKG MFDGEAPGQI GWSNELQQKT SSFGQSTTVT PVQMLQAQSA FFNDGNMLKP WFVNSVENPV SKRQFYKGQK QIAGKPITKD TAEKVEKQLD LVVNSKKSHA ANYRIDGYEV EGKTGTAQVA APNGGGYVKG PNPYFVSFMG DAPKKNPKVI VYAGMSLAQK NDQEAYELGV SKAFKPIMEN TLKYLNVGKS KDDTSNAEYS KVPDVEGQDK QKAIDNVSAK SLEPVTIGSG TQIKAQSIKA GNKVLPHSKV LLLTDGDLTM PDMSGWTKED VIAFENLTNI KVNLKGSGFV SHQSISKGQK LTEKDKIDVE FSSENVDSNS TNNSDSNSDD KKKSDSKTDK DKSD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCGAAGCAAAAAATTAAAATT  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTCCGACTTATCCTTGTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008