Clone

Target

IDP98971  

Clone Suffix

109

Sequence Comments

IDP98971.1-692.F..pMCSG53  

Experiment Start Date

Dec 16, 2021  

Experiment End Date

Dec 16, 2021  

Person

Michael Endres  

Lab

Argonne National Laboratory  

Protocol ID

standard cloning_ANL  

Status

failed  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

692  

Reference Sequence Used

MSAPAQPAPG VDGGDPSQAR IRVPAGTTAA TAVGEAGLPR RGTPDAIVVV RDADGNLRDL SWVPDVDTDI TPVAANTDDG RSVIRHSTAH VLAQAVQELF PQAKLGIGPP ITDGFYYDFD VPEPFTPEDL AALEKRMRQI VKEGQLFDRR VYESTEQARA ELANEPYKLE LVDDKSGDAE IMEVGGDELT AYDNLNPRTR ERVWGDLCRG PHIPTTKHIP AFKLTRSSAA YWRGDQKNAS LQRIYGTAWE SQEALDRHLE FIEEAQRRDH RKLGVELDLF SFPDEIGSGL AVFHPKGGIV RRELEDYSRR KHTEAGYQFV NSPHITKAQL FHTSGHLDWY ADGMFPPMHI DAEYNADGSL RKPGQDYYLK PMNCPMHCLI FRARGRSYRE LPLRLFEFGT VYRYEKSGVV HGLTRVRGLT MDDAHIFCTR DQMRDELRSL LRFVLDLLAD YGLTDFYLEL STKDPEKFVG AEEVWEEATT VLAEVGAESG LELVPDPGGA AFYGPKISVQ VKDALGRTWQ MSTIQLDFNF PERFGLEYTA ADGTRHRPVM IHRALFGSIE RFFGILTEHY AGAFPAWLAP VQVVGIPVAD EHVAYLEEVA TQLKSHGVRA EVDASDDRMA KKIVHHTNHK VPFMVLAGDR DVAAGAVSFR FGDRTQINGV ARDDAVAAIV AWIADRENAV PTAELVKVAG RE  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCCATGAGCGCCCCCGCACAAC  

PCR Backward Primer

TTATCCACTTCCAATGTTACTCACGACCGGCCACTTTCAC  

Shipped To Lab

ANL  

Shipped Date

Dec 16, 2021  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 11, 2022