Clone

Target

IDP00303  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 27, 2009  

Experiment End Date

Apr 29, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

424  

Reference Sequence Used

MNHRVVITGL GIIAPNANSV EHFESALRQG ISGIRHHSSL QQLNFLCQVA GTPQGVEAIA SRYFSEEQRL GMNSSMMYAS VAAVDCWRDA GFEYSQNDDH NHIDWTTGAI IGTGIGGMDT IGGELVPGVD AGRVRRLGST MVEQIMASAS SAKVGGLLGL GGQLSNVSSA CSSGTEAIAS AFYALKNGRA CRFLAGGVEG ASPYIWGGFD AMRVLAKGFN EAPEKASRPM SASASGFVPS AGAGLLMLET LESAEQRGAR IYAEILSASV NCGGQRQGGS MTAPNPLGVQ RCIQSAIEYA TISPSEIDAI NGHLTGTMAD KMELKNWATA LGCKPNELPW INSTKSLIGH SLGAAGGLEA VASVLQLNKG FIHGSLNCED IHPELEVFQD RIVQRTQEKQ IDVLAKASFG FGDVNACLIL KRWQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATCATCGTGTTGTGATTAC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTGCCAGCGTTTCAAAATC  

Shipped To Lab

UT  

Shipped Date

Jul 20, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2009