Clone

Target

IDP00304  

Clone Suffix

1

Sequence Comments

56: N -> D  

Experiment Start Date

Apr 27, 2009  

Experiment End Date

Apr 29, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

327  

Reference Sequence Used

MLYLHGLGHY HPNNIIDNSF LESLNINTNN EWILDRVGIH ERRTALPLNY IRETYNKNPT LAFQQMETSY ADGAARAVHH ALSRAGIETN QVGLVVAGEC ILQYTLPANA SVIAAAAGIQ SMAIDINTAC SSFATQMHYL NHLDESILPE YIVVVNPGFF TSIIDFTDRN TAVLFGDGAA AAVVSKRHES AWRLMDSHVD SNPKEWPVVT SPTGGHFSQQ GSRVQHFAIK TTVSEINYLS KRNAVNLNEI YFIGHQANLM MLQSAASRAG IAPEKHFYNV DRFGNCGAAS APSVFSQRWD SFKSNDQVLM SVVGAGLTWG SLLFKHL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MLYLHGLGHY HPNNIIDNSF LESLNINTNN EWILDRVGIH ERRTALPLNY IRETYDKNPT LAFQQMETSY ADGAARAVHH ALSRAGIETN QVGLVVAGEC ILQYTLPANA SVIAAAAGIQ SMAIDINTAC SSFATQMHYL NHLDESILPE YIVVVNPGFF TSIIDFTDRN TAVLFGDGAA AAVVSKRHES AWRLMDSHVD SNPKEWPVVT SPTGGHFSQQ GSRVQHFAIK TTVSEINYLS KRNAVNLNEI YFIGHQANLM MLQSAASRAG IAPEKHFYNV DRFGNCGAAS APSVFSQRWD SFKSNDQVLM SVVGAGLTWG SLLFKHL  

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTATATTTACACGGTCTTGG  

PCR Backward Primer

TTATCCACTTCCAATGTCATAGGTGCTTAAATAACAAAC  

Shipped To Lab

UT  

Shipped Date

Jul 20, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2009