Clone

Target

IDP00265  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2009  

Experiment End Date

Jul 24, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

356  

Reference Sequence Used

MGCVINWKGK ELKCGSGIFV TNEVHTWTEQ YKFQADSPKR VATAIAGAWE NGVCGIRSTT RMENLLWKQI ANELNYILWE NDIKLTVVVG DITGVLEQGK RTLTPQPMEL KYSWKTWGLA KIVTAETQNS SFIIDGPSTP ECPSASRAWN VWEVEDYGFG VFTTNIWLKL REVYTQLCDH RLMSAAVKDE RAVHADMGYW IESQKNGSWK LEKASLIEVK TCTWPKSHTL WSNGVLESDM IIPKSLAGPI SQHNHRPGYH TQTAGPWHLG KLELDFNYCE GTTVVISENC GTRGPSLRTT TVSGKLIHEW CCRSCTLPPL RYMGEDGCWY GMEIRPINEK EENMVKSLAS AGSGKV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGGGTGTGTCATAAACTGG  

PCR Backward Primer

TTATCCACTTCCAATGCACCTTTCCACTCCCTGCT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 10, 2009