Clone

Target
IDP90505  
Clone Suffix
1 - The clone is available in BEIR but it is out of stock.
Experiment Start Date
Oct 28, 2009  
Experiment End Date
Oct 28, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
118  
Reference Sequence Used
MARIAGINIP DQKHTVIALT AIFGIGKTRS QAICVAAGIA EHVKISELSE EQIEKLRDEV AKYVVEGDLR REVTLSIKRL MDLGTYRGLR HRRGLPVRGQ RTKTNARTRK GPRKPIKK  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MARIAGINIP DQKHTVIALT AIFGIGKTRS QAICVAAGIA EHVKISELSE EQIEKLRDEV AKYVVEGDLR REVTLSIKRL MDLGTYRGLR HRRGLPVRGQ RTKTNARTRK GPRKPIKK  
PCR Forward Primer
TACTTCCAATCCAATGCGATGGCCCGTATAGCAGGCA  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTCTTGATCGGTTTACGC  
Shipped To Lab
ANL  
Shipped Date
May 03, 2010  
Secondary Shipped To Lab
UT  
Secondary Shipped Date
Jun 19, 2012  
Inserted
Nov 04, 2009  

Related Expressions

       
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-04-02 2010-04-02 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2009-10-28 2009-10-28 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2010-08-02 2010-08-04 Min Zhou Argonne National Laboratory standard expression_ANL failed E. coli 2xYT 0.05% Rhamnose, 0.2% Arabinose, 0.5 mM IPTG production
2012-08-16 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production