Clone

Target
IDP90754  
Clone Suffix
1 - The clone is available in BEIR but it is out of stock.
Experiment Start Date
Oct 28, 2009  
Experiment End Date
Oct 28, 2009  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
258  
Reference Sequence Used
MILDKIFEKT KEDLKERKLK LPYDMLGRSL ASNPFFPKDV IKALKRVEKE VKIIAEVKKA SPSKGVIRED FDPLSIALNY EKNKAAAISV LTEPHFFKGS LEYLSLIRRY TQIPLLRKDF IFDEYQILEA LVYGADFVLL IAKMLSMKEL KKLLEFARHL GLEALVEIHD KEDLSKAIFA GADIIGINHR NLEDFTMDMS LCEKLIPQIP NSKIIIAESG LENKEFLEHL QNLGVDAFLI GEYFMREKDE GKALKALL  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MILDKIFEKT KEDLKERKLK LPYDMLGRSL ASNPFFPKDV IKALKRVEKE VKIIAEVKKA SPSKGVIRED FDPLSIALNY EKNKAAAISV LTEPHFFKGS LEYLSLIRRY TQIPLLRKDF IFDEYQILEA LVYGADFVLL IAKMLSMKEL KKLLEFARHL GLEALVEIHD KEDLSKAIFA GADIIGINHR NLEDFTMDMS LCEKLIPQIP NSKIIIAESG LENKEFLEHL QNLGVDAFLI GEYFMREKDE GKALKALL  
PCR Forward Primer
TACTTCCAATCCAATGCGATGATATTGGATAAAATTTTTGAAAA  
PCR Backward Primer
TTATCCACTTCCAATGTTAAAGCAAAGCTTTTAAAGCCT  
Shipped To Lab
ANL  
Shipped Date
May 03, 2010  
Inserted
Nov 04, 2009  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-08-02 2010-08-04 Min Zhou Argonne National Laboratory standard expression_ANL success E. coli 2xYT 0.05% Rhamnose, 0.2% Arabinose, 0.5 mM IPTG production
2009-10-28 2009-10-28 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2010-04-02 2010-04-02 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay