Clone

Target

IDP90784  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

434  

Reference Sequence Used

MNNHQTPQAT SPLAAWLCYL EHLHSQPIEL GLERVKQVAE RLDLLKPAPK IFTVAGTNGK GTTCCTLEAI LLAAGLRVGV YSSPHLLRYT ERVRIQGQEL SEAEHSHSFA QIEAGRGDIS LTYFEFGTLS ALQLFKQAKL DVVILEVGLG GRLDATNIVD SDVAAITSIA LDHTDWLGYD RESIGREKAG VFRGGKPAVV GEPDMPQSIA DVAAELGAQL YRRDVAWKFS QQEPFDQQEP VDQQINGWHW QCGERQLTGL PVPNVPLANA ATALAVLHYS ELPLSDEAIR QGLQAASLPG RFQVVSEQPL LILDVAHNPH AARYLVNRLA QVINPVNASK QGKVRAVVGM LSDKDIAGTL ACLSERVDEW YCAPLEGPRG ASAGQLAEHL VSARQFSDVE TAWRQAMQDA DTQDVVIVCG SFHTVAHVMA ALHL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MNNHQTPQAT SPLAAWLCYL EHLHSQPIEL GLERVKQVAE RLDLLKPAPK IFTVAGTNGK GTTCCTLEAI LLAAGLRVGV YSSPHLLRYT ERVRIQGQEL SEAEHSHSFA QIEAGRGDIS LTYFEFGTLS ALQLFKQAKL DVVILEVGLG GRLDATNIVD SDVAAITSIA LDHTDWLGYD RESIGREKAG VFRGGKPAVV GEPDMPQSIA DVAAELGAQL YRRDVAWKFS QQEPFDQQEP VDQQINGWHW QCGERQLTGL PVPNVPLANA ATALAVLHYS ELPLSDEAIR QGLQAASLPG RFQVVSEQPL LILDVAHNPH AARYLVNRLA QVINPVNASK QGKVRAVVGM LSDKDIAGTL ACLSERVDEW YCAPLEGPRG ASAGQLAEHL VSARQFSDVE TAWRQAMQDA DTQDVVIVCG SFHTVAHVMA ALHL  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAACAATCATCAAACTCCCC  

PCR Backward Primer

TTATCCACTTCCAATGTCACAAATGCAATGCTGCCAT  

Shipped To Lab

ANL  

Shipped Date

Nov 03, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009