Clone

Target

IDP90803  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

425  

Reference Sequence Used

MLLRLYQVLL YLIQPLIWLR LLLRSRKAPA YRKRWGERYG FCAGKVVAGG IMLHSVSVGE TLAAIPLVRA LRHRYPSLPI TVTTMTPTGS ERVQSAFGKD VHHVYLPYDL PGSVNRFLDQ VNPKLVIIME TELWPNLINT LHRRKIPLVI ANARLSARSA AGYKKIGSFI RTILQRITLI AAQNQEDGDR FIELGLKRSQ LTVTGSLKFD ISVTPELAAR AVTLRRQWAP HRPVWIATST HDGEETILLE AHRQLLQQFP TLLLILVPRH PERFGKAVEL TQKIGLSYTL RSKGEVPSSS TQVVIGDTMG ELMLLYGIAD LAFVGGSLVE RGGHNPLEAA AHAIPVLMGP HTFNFKDICA KLEQAEGLIT VTDTLSLVKE ITVLLTDEDC RLYYGRHAVD VLHENQGALQ RLLHLLEPYL PQRSH  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTGCTGCGTTTATACCAG  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTGGCTCCGTTGTGGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009