Clone

Target

IDP90821  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

317  

Reference Sequence Used

MDRNIIMTET PIRIATRQSP LALWQANYVK DALMAAHPGL QVELVTMVTR GDVILDTPLA KVGGKGLFVK ELEIAMLEGR ADLAVHSMKD VPVDFPDGLG LVTICEREDP RDAFVSNTYA KIEDLPSGAI VGTCSLRRQC QLKAARPDLV IKELRGNVGT RLSKLDAGEY DAIILAAAGL KRLELESRIR SFIEPEQSLP AVGQGAVGIE CRVNDQRVRA LLAPLNHADT ADRVRCERAM NLTLQGGCQV PIGSYALLEG DTIWLRALVG EPDGSQIVRG EIRGPRTQAE QLGITLAEQL LSQGAKEILE RLYCDHE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MDRNIIMTET PIRIATRQSP LALWQANYVK DALMAAHPGL QVELVTMVTR GDVILDTPLA KVGGKGLFVK ELEIAMLEGR ADLAVHSMKD VPVDFPDGLG LVTICEREDP RDAFVSNTYA KIEDLPSGAI VGTCSLRRQC QLKAARPDLV IKELRGNVGT RLSKLDAGEY DAIILAAAGL KRLELESRIR SFIEPEQSLP AVGQGAVGIE CRVNDQRVRA LLAPLNHADT ADRVRCERAM NLTLQGGCQV PIGSYALLEG DTIWLRALVG EPDGSQIVRG EIRGPRTQAE QLGITLAEQL LSQGAKEILE RLYCDHE  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGATAGGAACATCATAATGAC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTCGTGGTCACAGTAGAG  

Shipped To Lab

UT  

Shipped Date

Nov 04, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009