Clone

Target

IDP90908  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

269  

Reference Sequence Used

MFLINGHAQD QLAVSDRATQ FGDGSFTTAR IVDGNICHLE AHLQRLQVAC EKLRIAFSHW STLRQEMTML ATGHDSGVLK VIISRGSGGR GYSAMNCQAA TRILSVSAYP AYYSQWRKQG ITLTLSPIPL GRNPYLAGLK HLNRLEQVLI RSHLEQTDAD EALVLDSEGW VTECCAANLF WRTGDIVFTP RLDQAGVNGI MRQFCLRQLA QSPFQVLEVQ AREEAVRQAD EIIICNALMP IIPIRAYHGT SYSSRTLFQF LAPFCEHPN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MFLINGHAQD QLAVSDRATQ FGDGSFTTAR IVDGNICHLE AHLQRLQVAC EKLRIAFSHW STLRQEMTML ATGHDSGVLK VIISRGSGGR GYSAMNCQAA TRILSVSAYP AYYSQWRKQG ITLTLSPIPL GRNPYLAGLK HLNRLEQVLI RSHLEQTDAD EALVLDSEGW VTECCAANLF WRTGDIVFTP RLDQAGVNGI MRQFCLRQLA QSPFQVLEVQ AREEAVRQAD EIIICNALMP IIPIRAYHGT SYSSRTLFQF LAPFCEHPN  

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTTTTAATTAATGGCCATGC  

PCR Backward Primer

TTATCCACTTCCAATGCTAATTCGGATGCTCACAAAAT  

Shipped To Lab

ANL  

Shipped Date

May 03, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009