Clone

Target

IDP04356  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

540  

Reference Sequence Used

MPPQIDRRYF QMAPPGQWLK KSAWPDSVPF KLIGFRPDNE EISGAFPAVS ARAFVWDNPS APPSEVTLLR KTLWLLPDND MGLMVFTGSV PLTHLFDEPI DTLLVGLDDS HSLRELEYYQ QVYKSRSVEG AASFEFLKDP ELMPEGMPLN VIRDLADHPD SLRYSASAMS EAESERFYQD VQDAIDRQEQ QKSEEQETLG DLNVPAAGKE EAGTQWLESK EDTATNVTFL GTDFSGMTLD NKQFRYCMFT GCHFDKATFK DCTFEHCQFT QSDFENSRWN NVHLSGCLFK QAEWQKAAFT HCKWEKSTFE YGVFKHAQFT DNALDNCLIN HSDFSLGTFD HCTLNGCFFS ETHCDQTQFN QVIITSCIFE KCDGPKACFT ESTIEKTSFI SSSWVGGRLS HCYLNSLTTG LNTNLSESHF EQCSLNKMGF LKVNLQSSTF INCSMLESCC DKADFSQATL IACDMTAVRL KDANLVHSHW QNTSLQQSMF YNADLRDATF QRCNLAGANL AMISQNMDTR FEHCLTEKTH WIPRRYTVPA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCCACCGCAAATTGATCG  

PCR Backward Primer

TTATCCACTTCCAATGTTATGCAGGGACGGTGTAAC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 03, 2009